Work

Cutting Edge: Effects of an Allergy-Associated Mutation in the Human IL-4Rα (Q576R) on Human IL-4-Induced Signal Transduction

Year: 1999

Type: article

Abstract: A mutation in the human (hu) IL-4R alpha, Q576R, has been linked with allergy in humans. Increased sensitivity of patients cells with this mutation to IL-4 suggest that a Q576R change enhances IL-4 signaling. To directly test this hypothesis, we analyzed the ability of huIL-4R alpha cDNA bearing the Q576R and Y575F mutations to signal tyrosine phosphorylation, DNA-binding activity, proliferation, protection from apoptosis, and CD23 induction in response to huIL-4 in murine cells. Responses generated by the Q576R and Y575F mutants were similar to those of the wild-type receptor, using various concentrations of huIL-4 and times of stimulation. These results indicate that neither the Q576R nor the Y575F mutations have a significant direct effect on IL-4 signal transduction, and that hypersensitive induction of CD23 in cells derived from human allergy patients may be due to different and/or additional alterations in the IL-4 signaling pathway.

Source: The Journal of Immunology

DOI: 10.4049/jimmunol.162.8.4385

Authors: Helen Y Wang, Chris P Shelburne, Jose Zamorano, Ann E Kelly, John J Ryan, Achsah D Keegan

Institutions: American Red Cross, Virginia Commonwealth University

Language: English

Topic: Cytokine Signaling Pathways and Interactions

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,500