Work

19F NMR quantitation of lens aldose reductase activity using 3-deoxy-3-fluoro-D-glucose.

Year: 1991

Type: article

Abstract: In the present study we have determined the kinetics of 3-deoxy-3-fluoro-D-glucose (3-FG) as a substrate for the aldose reductase reaction in vitro. In addition, we compared the 3-deoxy-3-fluoro-sorbitol (3-FS) production rates from 3-FG in the intact lens using 19F NMR with conventional aldose reductase determinations in extracts from the same lenses. The affinity of in vitro aldose reductase for 3-FG was approximately 20 times greater (9.3 mM) than that for glucose (188 mM). An excellent correlation between the rate of 3-FS production in the intact canine lens, determined with 19F NMR, and extracted aldose reductase activity was observed. The relatively high affinity of aldose reductase for 3-FG and the correlation of 3-FS production with enzyme activity in the intact lens suggests that 3-FS production from 3-FG detected by 19F NMR could provide an accurate noninvasive determination of aldose reductase activity in the eye lens.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(18)54806-7

Authors: K. Karino, P.F. Kador, B. Berkowitz, R.S. Balaban

Institutions: National Institutes of Health, National Heart, Lung, and Blood Institute

Language: English

Topic: Aldose Reductase and Taurine

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,430