Work

Nonisotopic oligomeric probes for the human enteroviruses

Year: 1988

Type: article

Abstract: Oligonucleotide probes were prepared from highly conserved regions of human enteroviral genomes. These reagents were labeled with either 32P or alkaline phosphatase and were successfully used in blot assays to detect a wide variety of human enteroviruses, proving the potential utility of oligomeric sequences as pan-enteroviral probes. The availability of nonisotopic probes will ultimately make a hybridization assay for enteroviruses easier, shorter, and more adaptable for routine diagnostic laboratories.

Source: Journal of Clinical Microbiology

DOI: 10.1128/jcm.26.12.2669-2671.1988

Authors: H A Rotbart, P S Eastman, J L Ruth, K K Hirata, M J Levin

Institutions: University of Colorado Anschutz, University of Colorado Denver

Language: English

Topic: Viral Infections and Immunology Research

Subfield: Cardiology and Cardiovascular Medicine

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of Allergy and Infectious Diseases, National Institute of Diabetes and Digestive and Kidney Diseases

Awards: G2786614669, DIAGNOSIS &PATHOGENESIS OF HUMAN ENTEROVIRUS INFECTIONS, G5500101199