Effect of potential prebiotics on the survival of probiotic Lactobacillus acidophilus.
Year: 2009
Type: article
Abstract: Four locally available sources of Arrowroot (Maranta arundinacea L.), Hingurala (Dioscorea alata) Innala (Plectranthus rotundifolius), rice bran and commercially available raftiline ® were tested for their prebiotic quality in obtaining increased biomass of probiotic Lactobacillus acidophilus. These sources were incorporated at two levels (1 and 3%) into sterile skim milk containing 12% (w/v) solids at two stages of the incubation process, the two stages being at the time of inoculation and 1 h after incubation. The inoculum used was one g of prepared culture containing 10 9 - 10 10 colony forming units /g of Lactobacillus acidophilus. All the samples were incubated at 37 o C anearobically for 12 h followed by refrigeration at 4 o C. Plate counts and pH of each sample were measured throughout the storage period from 0, 3, 7, 14, 21, and 28 days. All the sources showed significant increase in the colony forming units compared to the control while arrow root and hingurala showed best results with 7.71 and 7.55 log 10 /g respectively at the end of 28 days of storage. All the sources showed significant counts when added one hour after incubation. The control showed highest acidity at the end of storage (pH 4.11) while Innala showed the lowest (pH 4.32). The survival of Lactobacillus acidophilus was generally enhanced by all the potential prebiotics while maintaining the standards required for a probiotic.
Source: Tropical Agricultural Research
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W1531659141