Work

Activity‐Based Protein Profiling (ABPP) and Click Chemistry (CC)–ABPP by MudPIT Mass Spectrometry

Year: 2009

Type: article

Abstract: Activity‐based protein profiling (ABPP) is a chemical proteomic method for functional interrogation of enzymes within complex proteomes. This unit presents a protocol for in vitro and in vivo labeling using ABPP and Click Chemistry (CC)‐ABPP probes for in‐depth profiling using the Multi‐dimensional Protein Identification Technology (MudPIT) analysis platform. Curr. Protoc. Chem Biol. 1:29‐41. © 2009 by John Wiley & Sons, Inc.

Source: Current Protocols in Chemical Biology

DOI: 10.1002/9780470559277.ch090138

Authors: Anna E. Speers, Benjamin F. Cravatt

Institution: Scripps Research Institute

Language: English

Topic: Click Chemistry and Applications

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Cancer Institute

Award: G2948931604