Effects of exogenous jasmonic acid on the accumulation of glyceollins and glycinol in Glycine max (L.) Merri
Year: 2005
Type: other
Abstract: Health cotyledons were detached from 8-day-old soybean seedlings (Zhonghuang No. 13). A section of tissue, approximately 2×2mm was cut from the concave surface. 50#mu#l jasmonic acid or silver nitrate solution was dripped into the wound and incubation in the dark at 25℃. Glyceollins and glycinol were extracted by methanol and analyzed by HPLC. The results show that there is no accumulation of glyceollins and glycinol in health cotyledons. Silver nitrate acts much stronger than jasmonic acid to induce the accumulation of glyceollins and glycinol. The accumulation of glyceollins is in inverse proportion to the concentration of jasmonic acid. The percent of 3 glyceollin isomer is 50%-80%, 10%-40% and 10%-20% for glyceollin Ⅰ, Ⅱ and Ⅲ, respectively. During the incubation, the ratio of glyceollin Ⅰ decreases and that of glyceollin Ⅱ increases. It can be obviously observed in samples treated by silver nitrate.
Authors: Y. Qi, Arnaud Bovy, C.H. de Vos, Yueming Tang
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W1532423476