pH dependence of the reoxidation of p-hydroxybenzoate hydroxylase 2,4-dihydroxybenzoate complex.
Year: 1984
Type: article
Abstract: Oxidation of reduced p-OH-benzoate hydroxylase . 2,4-diOH-benzoate complex by molecular oxygen occurs in four clearly defined steps. Inclusion of azide in the reaction medium slows the rate for each step. We have found that the rates for the last three steps as well as the UV/visible absorbance spectrum for the second intermediate are markedly dependent on pH. Formation of intermediate II is base-catalyzed while its decay to intermediate III is acid-catalyzed. Formation of oxidized enzyme from intermediate III is base-catalyzed. The spectrum of intermediate II is shifted to longer wavelengths on raising the pH. A pKa of about 7.8 can be calculated for each of these pH-dependent transitions, except for the rate of formation of oxidized enzyme. This rate appears to be directly dependent on hydroxide ion concentration. The presence of azide does not change the observed pH dependence. These results are discussed in the context of a 4a,5-ring opened flavin structure for intermediate II.
Source: Journal of Biological Chemistry
Authors: A Wessiak, L M Schopfer, V Massey
Institution: University of Michigan
Language: English
Subfield: Inorganic Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W1534619350