Work

Rapid Production of Graphite Without Contamination for Biomedical AMS

Year: 1992

Type: article

Abstract: The application of AMS to the detection of 14C makes possible a new class of sensitive experiments in molecular biology. Such experiments inherently produce large numbers of samples for the determination of biological variability in molecular interactions. The samples vary in 14C concentration over many orders of magnitude. We added TiH2 to aid the reduction of CO2 by zinc in a sealed tube to reproducibly make graphite without sample cross-contamination. The CO2 is transferred from a combustion tube to the reaction tube through a disposable plastic manifold. The sealed tubes are heated to a single-reaction temperature in a muffle furnace. The process is complete within 5 h. Bulk isotopic fractionation in the finished graphite is less than 0.5%.

Source: Radiocarbon

DOI: 10.1017/s0033822200063529

Author: John S. Vogel

Institution: Lawrence Livermore National Laboratory

Language: English

Topic: Archaeology and ancient environmental studies

Subfield: Archeology

Field: Social Sciences

Domain: Social Sciences

Open Access status: bronze

Funders: U.S. Department of Energy, Lawrence Livermore National Laboratory

Awards: G1702950523, G62358869, G6445123630, G660437508