Work

Identification of cDNAs encoding two G protein‐coupled receptors for lysosphingolipids1

Year: 1997

Type: article

Abstract: The structural similarity of lysosphingolipids to lysophosphatidic acid (LPA) prompted a sequence-based search for lysosphingolipid receptors using cDNA sequence of the Edg2 human LPA receptor. Two closely related G protein-coupled receptors, rat H218 and human Edg3, are highly similar to Edg2. When overexpressed in Jurkat cells, H218 and Edg3 activated serum response element-driven transcriptional reporter gene in response to sphingosine 1-phosphate (S1P), dihydro-S1P and sphingosylphosphorylcholine, but not to LPA. H218 and Edg3 expressed in Xenopus oocytes conferred responsiveness to S1P and dihydro-S1P in agonist-triggered 45Ca2+ efflux. Therefore, H218 and Edg3 are functional receptors for S1P and perhaps other closely related lysosphingolipids.

Source: FEBS Letters

DOI: 10.1016/s0014-5793(97)01301-x

Authors: Songzhu An, Thieu Bleu, Wei Huang, Olivia G Hallmark, Shaun R Coughlin, Edward J Goetzl

Institution: University of California, San Francisco

Language: English

Topic: Sphingolipid Metabolism and Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: National Heart, Lung, and Blood Institute

Awards: LYSOLIPID PHOSPHATE REGULATION OF LYMPHOCYTE FUNCTIONS, Molecular Mechanisms of Protease Signaling