Work

Introduction of shRNAs into Human NK-Like Cell Lines with Retrovirus

Year: 2009

Type: book-chapter

Abstract: Natural killer (NK) cell lines are difficult to transfect using standard techniques, which limits the ability to establish long-term knockdown of proteins with short-hairpin (sh)RNAs. We have developed a method to stably knockdown protein expression in human NK-like lines by introducing shRNAs in retroviral vectors. After a single transduction with retrovirus, shRNA-containing cells can be selected with drug treatment or sorted for enhanced green fluorescent protein (EGFP) expression. With this method, protein expression can be stably decreased to less than 10% of wild-type levels.

Source: Methods in molecular biology

DOI: 10.1007/978-1-60761-362-6_15

Authors: Amanda K. Purdy, Kerry S. Campbell

Institution: Fox Chase Cancer Center

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Cancer Institute