Work

Sequence-specific Binding of Arc Repressor to DNA

Year: 1989

Type: article

Abstract: A set of arc operators with transition and/or transversion mutations at each operator base pair has been constructed. By determining the ability of Arc to bind these variant operators, the importance of each base pair for Arc recognition has been assessed. Methylation protection experiments have also been used to probe points of close contact between Arc and most of the mutant operators. These data, together with phosphate interference results obtained previously for the wild type operator, provide information about the operator surface that is contacted when Arc binds.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(18)94062-7

Authors: A K Vershon, R D Kelley, R T Sauer

Institution: Massachusetts Institute of Technology

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

Funder: National Institute of Allergy and Infectious Diseases