The Identity of a Hydroxylamine-sensitive Bond in the α1 Chain of Rat Skin Collagen
Year: 1969
Type: article
Abstract: A peptide obtained by treatment of the α1 chain of rat skin collagen with cyanogen bromide, α1-CB3 (149 amino acids), was cleaved with hydroxylamine. Partial characterization of the products indicated that the cleavage was at two sites near the COOH terminus of the peptide. Comparative amino acid analyses of one of these products and of peptides isolated from a tryptic digest of α1-CB3 have allowed the identification of a small peptide (12 amino acids) from the trypsin hydrolysate with an intact hydroxylamine-sensitive bond. Edman degradation of the appropriate peptides provided evidence that at least one hydroxylamine-sensitive bond of collagen is a peptide (Asn-Gly) bond.
Source: Journal of Biological Chemistry
Author: W T Butler
Institution: University of Alabama at Birmingham
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W1538814084