Heterogeneity of Nucleolar U3 Ribonucleic Acid of the Novikoff Hepatoma
Year: 1971
Type: article
Abstract: Among the low molecular weight RNAs which have been shown to be localized to the nucleolus are the 4 S, U3, and 8 S RNAs. Of these, the U3 and 8 S RNAs are hydrogen bonded to nucleolar 28 S RNA. The nucleolar U3 RNA migrates as an electrophoretically homogeneous band on 8% polyacylamide gels but was separated into four bands on 16% gels. Although these bands have similar nucleotide compositions, chemical analysis of the hydroxymethyl ethylene glycol derivatives of the 3'-terminal nucleosides produced by periodate oxidation and reduction with tritiated potassium borohydride has shown that these bands contain several components. Separation on DEAE-Sephadex of the oligonucleotides obtained by T1 and pancreatic RNase digestion showed that three different groups of terminal oligonucleotides are present. The nucleoside peak contains 3'-terminal guanosine, 85%, and cytidine, 15%. The mononucleotide peak contains terminal adenosine, 62%, uridine, 26%, and cytidine, 8%. The hexanucleotide peak contains terminal uridine, 100%. On the basis of the enzymatic digestions employed, the terminal oligonucleotides are PyG, PyC, PyGAA, PyGAU, PyGAC, PyG(Np)4NmpNmpU.
Source: Journal of Biological Chemistry
Authors: Archie W. Prestayko, Maurizio Tonato, B. Carol Lewis, Harris Busch
Institution: Baylor College of Medicine
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W1542080395