Work

Phosphoproteomics technologies and applications in plant biology research

Year: 2015
Type: review
Abstract: Protein phosphorylation has long been recognized as an essential mechanism to regulate many important processes of plant life. However, studies on phosphorylation mediated signaling events in plants are challenged with low stoichiometry and dynamic nature of phosphorylated proteins. Significant advances in mass spectrometry based phosphoproteomics have taken place in recent decade, including phosphoprotein/phosphopeptide enrichment, detection and quantification, and phosphorylation site localization. This review describes a variety of separation and enrichment methods for phosphoproteins and phosphopeptides, the applications of technological innovations in plant phosphoproteomics, and highlights significant achievement of phosphoproteomics in the areas of plant signal transduction, growth and development.
Language: English
Subfield: Spectroscopy
Field: Chemistry
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W1542173032
FWCI: 0.478
Cites: 68
Cited by: 46
Related to: 10