Work

Engineering Three‐Dimensional Collagen Matrices to Provide Contact Guidance during 3D Cell Migration

Year: 2010

Type: article

Abstract: Cell invasion requires that cells navigate complex three-dimensional matrices in vivo. Topological cues are provided and three-dimensional cell migration and invasion facilitated by the alignment of collagen fibers proximal to tumors. In order to better understand the molecular mechanisms by which cells recognize and migrate along aligned matrices, in vitro assays are needed that recapitulate topological features of the in vivo matrix. Here, we describe two approaches for creating aligned three-dimensional collagen matrices, both dependent and independent of cell-mediated alignment. Approaches to quantify alignment and visualize the collagen matrix relative to the cells by second-harmonic generation are included. These assays are readily adaptable to a variety of cells and biological questions related to three-dimensional cell migration.

Source: Current Protocols in Cell Biology

DOI: 10.1002/0471143030.cb1017s47

Authors: Paolo P. Provenzano, Kevin W. Eliceiri, David R. Inman, Patricia J. Keely

Institutions: University of Wisconsin–Madison, Fred Hutch Cancer Center

Language: English

Topic: Cellular Mechanics and Interactions

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Cancer Institute

Awards: Development of The Prairie Technologies MPLSM to image cancer models in vivo, G3592001316, G5468130066, Mechanisms Of Cell Migration On 3D Aligned Matrices