Work

Biosynthesis of Peptidoglycan in the One Million Molecular Weight Range by Membrane Preparations from Bacillus megaterium

Year: 1974

Type: article

Abstract: Peptidoglycan made in vitro by membrane preparations of Bacillus megaterium had a buoyant density in CsCl different from that of native cell wall. This newly made material was not covalently attached to substantial amounts of previously formed cell material such as wall. From sedimentation velocity centrifugation, the molecular weight of much of the in vitro product was found to be greater than 6 x 106. Peptidoglycan made in the presence of penicillin had smaller molecular weights suggesting that transpeptidation was in part responsible for the large size of the product. With reaction conditions in which transpeptidation was presumed to have occurred, release of alsnine was observed. This release was inhibited by penicillin.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(19)42392-2

Authors: William P. Schrader, Bruce E. Beckman, Mary M. Beckman, John S. Anderson, David P. Fan

Institution: University of Minnesota

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1543645503

FWCI: 1.596

Cites: 10

Cited by: 10

Related to: 10