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Adenosine 3′,5-Monophosphatedependent Phosphorylation of a Specific Protein in Synaptic Membrane Fractions from Rat Cerebrum

Year: 1972

Type: article

Abstract: The endogenous phosphorylation of protein in various membranous subcellular fractions from rat cerebrum has been studied in vitro. The endogenous phosphorylation of one minor protein component of synaptic membrane fractions showed a marked dependence upon the presence of added adenosine 3',5'-monophosphate (cyclic AMP). The apparent molecular weight of this phosphoprotein was approximately 100,000. Under the experimental conditions used, the selective stimulation of phosphorylation of a membrane protein by cyclic AMP was observed only in those subcellular fractions enriched in synaptic membranes. The results support other evidence suggesting that certain types of synaptic transmission may involve cyclic AMP-dependent phosphorylation of a specific protein constituent of synaptic membranes.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(20)81153-3

Authors: Edward M. Johnson, Tetsufumi Ueda, Hiroo Maeno, Paul Greengard

Institution: Yale University

Language: English

Topic: Neuroscience and Neuropharmacology Research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1544639113

FWCI: 9.077

Cites: 10

Cited by: 176

Related to: 10