Work

On the Mechanism of the Aconitase and Isocitric Dehydrogenase Reactions

Year: 1957

Type: article

Abstract: The following abbreviations will be used: DPN, diphosphopyridine nucleotide; TPN, triphosphopyridine nucleotide; DPNH and TPNH, the reduced forms; DPND and TPND, reduced deuterio-DPN and reduced deuterio-TPN.2 In the experiments of San Pietro et al. (1) the added DPN was only partially reduced by the isocitric dehydrogenase-transhydrogenase system.Labeling apparently resulted from incorporation of deuterium into the remaining DPN during the boiling period after the enzyme reaction had been stopped.This interpretation is based on the following observations made during the course of the present work.1047

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(18)70889-2

Authors: Sasha Englard, Sidney P. Colowick

Institutions: Albert Einstein College of Medicine, Johns Hopkins University, Yeshiva University

Language: English

Topic: Biochemical Acid Research Studies

Subfield: Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1544948283

FWCI: 2.838

Cites: 27

Cited by: 47

Related to: 10