Work

Immunologic detection of a protein homologous to chicken progesterone receptor B subunit.

Year: 1985

Type: article

Abstract: A monoclonal antibody described previously by us (Edwards, D. P., Weigel, N. L., Schrader, W. T., O'Malley, B. W., and McGuire, W. L. (1984) Biochemistry 23, 4427-4435) was used to study progesterone receptor B subunits of chick and hen oviduct. We find that the antibody does not recognize the form of receptor B able to bind [3H]progesterone in vitro. Rather, it reacts exclusively with a homologous protein of the same molecular weight, termed B antigen. The antigen is present in both immature estrogen-treated chicks and in egg-laying hens. This antigen is indistinguishable from the hormone-binding receptor species (termed receptor B) as shown by peptide mapping techniques using either Staphylococcus aureus V8 protease or trypsin. The B antigen and the hormone binder can be resolved by ion-exchange chromatography. Sedimentation velocity data show that the two proteins are present in distinct, separable cytosolic entities. The functional relationship between the two proteins has not been established.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(17)39499-1

Authors: S Peleg, W T Schrader, D P Edwards, W L McGuire, B W O'Malley

Institutions: The University of Texas at San Antonio Health Science Center, Baylor College of Medicine

Language: English

Topic: Estrogen and related hormone effects

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

Funder: Eunice Kennedy Shriver National Institute of Child Health and Human Development