Release of enzymes from lysosomes by irradiation and the relation of lipid peroxide formation to enzyme release
Year: 1966
Type: article
Abstract: 1. Acid phosphatase, cathepsin and beta-glucuronidase are released from rat-liver lysosomes by irradiation in vitro. Enzyme release is detectable after a dose of 1krad and increases with dose up to 100krads. 2. Maximum radiation effects were observed when the lysosomes were kept for 20hr. at 4 degrees or 20 degrees after irradiation. 3. An atmosphere of nitrogen considerably decreases enzyme release from lysosomes. 4. Enzyme release is enhanced by ascorbic acid and decreased by vitamin E. 5. Irradiation causes formation of lipid peroxides in lysosomes, and enzyme release increases with lipid peroxide formation. 6. It is suggested that lipid peroxide formation leads to rupture of the lysosome membrane and allows release of the contained hydrolytic enzymes.
Source: Biochemical Journal
DOI: 10.1042/bj0990657
Authors: ED Wills, AE Wilkinson
Institutions: St Bartholomew's Hospital, Queen Mary University of London
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W1548153124