Preparation and Initial Biocompatability Evaluation of Biogenic Hydroxyapatite/ Chitosan/ Pva Biocomposite as a Drug Delivery Carrier of Proneurogenic Factor
Year: 2015
Type: article
Abstract: Objective: This study explores the synthesis of biogenic hydroxyapatite (HAp)/chitosan (CS)/polyvinyl alcohol (PVA) bio-composites for delivery of proneurogenic factor, retinoic acid for reconstruction of craniofacial deformities. Methods: In order to accomplish this aim, we started with the synthesis of HAp using the biomolecules occluded in the cucumber peel (CPHAp). Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction studies (XRD) studies confirmed the purity and morphology of CPHAp. Further, a microenvironment for nerve cell growth was designed by synthesis of biogenic HAp/CS/PVA blends loaded with retinoic acid (CS-PVA-CPHAp-all-trans-retinoic acid [ATRA]). The prepared biocomposites were characterized under advanced analytical instruments such as SEM, FTIR, and XRD. Results: The SEM analysis for the prepared biocomposites confirmed the formation of interconnected porous matrix. The results of FTIR confirm the biocomposite formation without chemical modification of ATRA. From XRD the amorphous nature was confirmed, inducing suitability of the material for delivery process. Release of ATRA from CS-PVA-CPHAp-ATRA was sustained, with a cumulative release of 55% at the end of 10th day. Furthermore, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay indicated that, the biocomposites are better in scaffold properties, and it provides a healthier environment for cell attachment and spreading. Conclusion: The porous CS-PVA CPHAp-ATRA composites may be applied to craniofacial tissue engineering as a long-term or permanent scaffold due to their good biocompatibility and sustained release of proneurogenic factor. Keywords: Hydroxyapatite/chitosan/polyvinyl alcohol bio-composites, Biocompatibility, Cell compatibility, Scaffold, Craniofacial deformities.
Institution: Sathyabama Institute of Science and Technology
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W1548442219