Optimization of oligonucleotide arrays and RNA amplification protocols for analysis of transcript structure and alternative splicing
Year: 2003
Type: article
Abstract: Microarrays offer a high-resolution means for monitoring pre-mRNA splicing on a genomic scale. We have developed a novel, unbiased amplification protocol that permits labeling of entire transcripts. Also, hybridization conditions, probe characteristics, and analysis algorithms were optimized for detection of exons, exon-intron edges, and exon junctions. These optimized protocols can be used to detect small variations and isoform mixtures, map the tissue specificity of known human alternative isoforms, and provide a robust, scalable platform for high-throughput discovery of alternative splicing.
Source: Genome biology
Institution: Merck & Co., Inc., Rahway, NJ, USA (United States)
Language: English
Topic: RNA Research and Splicing
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,490
OpenAlex ID: W1549698322