Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels
Year: 2006
Type: article
Abstract: A reliable, fast, cheap and sensitive silver staining method to detect nucleic acids in polyacrylamide gels was developed from two standard stain procedures.The main differences between the three methods concerned (i) the preexposure with formaldehyde during silver nitrate impregnation, (ii) the addition of sodium thiosulfate and sodium carbonate instead of sodium hydroxide during development; (iii) the removal of the stop reaction or the inclusion of absolute ethanol with acetic acid in the stop solution and (iv) the duration of the different reaction steps.All methods allowed the detection of similar polymorphisms for single sequence repeats with different cotton genotypes but important differences regarding the contrast, background and conservation duration of the gels were observed.Two methods gave superior sensitivity.The improved method was sensitive, fast (20 min), gave lower backgrounds, produced gels with long-term conservation ability, and allowed a reutilization of all the solutions used in the staining procedure from fi ve to seven times, making it quite cheap.
Source: ORBi (University of Liège)
Authors: Mergeai G., Baudoin JP., Jacquemin JM., Benbouza H.
Institutions: University of Liège, Gembloux Agro-Bio Tech
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W1551072555