Biosynthesis of the Peptidoglycan of Bacterial Cell Walls
Year: 1970
Type: article
Abstract: The enzyme which catalyzes the transfer of l-alanine from l-alanyl transfer RNA to UDP-N-acetylmuramyl-l-Ala-d-Glu-l-Lys-d-Ala-d-Ala has been purified to homogeneity, as shown by polyacrylamide gel electrophoresis, from an extract of Lactobacillus viridescens. This enzyme has a molecular weight of 40,000 as judged from gel electrophoresis. It also catalyzes transfer reactions involving l-seryl-tRNA, l-cysteinyl-tRNA and, to some extent, glycyl-tRNA and will also utilize l-alanyl-tRNAcys prepared by reduction of l-cysteinyl-tRNA with Raney nickel. It thus has virtually no specificity for the tRNA molecule involved in the transfer reaction. It has a somewhat higher, although not strict specificity, for the amino acid transferred.
Source: Journal of Biological Chemistry
Authors: Roland Plapp, Jack L. Strominger
Institution: Harvard University
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W1552720336