Work

Production of beta-carotene in Zymomonas mobilis and Agrobacterium tumefaciens by introduction of the biosynthesis genes from Erwinia uredovora

Year: 1991

Type: article

Abstract: The Erwinia uredovora crtB, crtE, crtI, and crtY genes required for beta-carotene biosynthesis were introduced by conjugal transfer into an ethanol-producing bacterium, Zymomonas mobilis, and a phytopathogenic bacterium, Agrobacterium tumefaciens, in which no carotenoid is synthesized. The transconjugants of Z. mobilis and A. tumefaciens carrying these genes appeared as yellow colonies and produced 220 and 350 micrograms of beta-carotene per g of dry weight, respectively, in the stationary phase in liquid culture.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.57.6.1847-1849.1991

Authors: N Misawa, S Yamano, H Ikenaga

Institutions: Kirin (Japan), Kirin Brewery Company, Limited (Japan)

Language: English

Topic: Microbial Metabolism and Applications

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: bronze

OpenAlex ID: W1552989068