Year: 1982
Type: article
Abstract: Barbiturates enhance GABA receptor (sodium-independent) binding at 0°C to bovine brain membranes about 2-fold in chloride-containing buffer but not in chloride-free phosphate or Tris/ citrate buffer.This effect is observed in frozen and thawed, thoroughly washed membranes from various regions of bovine brain as well as washed membranes prepared from unfrozen rat cerebral cortex; it is destroyed by brief treatment of the membranes with the detergent Triton X-100.The enhancement is concentration-dependent, with approximately 250 pM pentobarbital giving 50% of the maximal effect seen with saturating 2 mM barbiturate.This effect is reversed by the GABA from Hoffmann-LaRoche, Inc.
Source: Journal of Neuroscience
Authors: RW Olsen, AM Snowman
Institution: University of California, Riverside
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W1554442106