Adrenergic and serotonergic function in dna synthesis during rat liver regeneration and in hepatocyte cultures
Year: 1997
Type: dissertation
Abstract: The adult mammalian liver is predominantly in a quiescent state with respect to cell\ndivision. This quiescent state changes dramatically, however, if the liver is injured by\ntoxic, infectious or mechanic agents (Ponder, 1996). Partial hepatectomy (PH) which\nconsists of surgical removal of two-thirds of the liver, has been used to stimulate\nhepatocyte proliferation (Higgins & Anderson 1931). This experimental model of liver\nregeneration has been the target of many studies to probe the mechanisms responsible for\nliver cell growth control (Michalopoulos, 1990; Taub, 1996). After PH most of the\nremaining cells in the renmant liver respond with co-ordinated waves of DNA synthesis\nand divide in a process called compensatory hyperplasia. Hence, liver regeneration is a\nmodel of relatively synchronous cell cycle progression in vivo. In contrast to hepatomas,\ncell division is terminated under some intrinsic control when the original cellular mass has\nbeen regained. This has made liver regeneration a useful model to dissect the biochemical\nand molecular mechanisms of cell division regulation. The liver is thus, one of the few\nadult organs that demonstrates a physiological growth rewonse (Fausto & Mead, 1989;\nFausto & Webber, 1994). The regulation of liver cell proliferation involves circulating or\nintrahepatic factors that are involved in either the priming of hepatocytes to enter the cell\ncycle (Go to G1) or progression through the cell cycle. In order to understand the basis of\nliver regeneration it is mandatory to define the mechanisms which (a) trigger division, (b)\nallow the liver to concurrently grow and maintain dilferentiated fimction and (c) terminate\ncell proliferation once the liver has reached the appropriate mass. Studies on these aspects\nof liver regeneration will provide basic insight of cell growth and dilferentiation, liver\ndiseases like viral hepatitis, toxic damage and liver transplant where regeneration of the\nliver is essential. In the present study, Go/G1/S transition of hepatocytes re-entering the\ncell cycle after PH was studied with special emphasis on the involvement of\nneurotransmitters, their receptors and second messenger function in the control of cell\ndivision during liver regeneration
Authors: B Sudha, C.S. Paulose
Language: English
Subfield: Hepatology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W1554459196