Work

Characterization of the asialoglycoprotein receptor in a continuous hepatoma line.

Year: 1981

Type: article

Abstract: The asialoglycoprotein receptor has been identified on a continuous human hepatoma cell line, HepG2. This receptor requires Ca2+ for ligand binding and is specific for asialoglycoprotein. There are approximately 150,000 ligand molecules bound/cell at 4 degrees C. These receptors represent a homogeneous population of high affinity binding sites with Kd = 7 X 10(-9) M. From the rate of 125I-ASOR binding at 4 degrees C, kon was 0.95 X 10(6) M-1 min-1. Uptake of 125I-ASOR at 37 degrees C was approximately 0.02 pmol/min/10(6) cells.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(19)52477-2

Authors: A L Schwartz, S E Fridovich, B B Knowles, H F Lodish

Institutions: The Wistar Institute, Harvard University, Massachusetts Institute of Technology

Language: English

Topic: Glycosylation and Glycoproteins Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,430