Work

Synthesis of Peptide‐Oligonucleotide Conjugates Using a Heterobifunctional Crosslinker

Year: 2010

Type: article

Abstract: Peptide-oligonucleotide conjugates (POCs) are molecular chimeras composed of a nucleic acid moiety covalently attached to a polypeptide moiety. POCs have been used in numerous applications from therapeutics to nanotechnology, and most recently as combinatorial agents in the assembly of bivalent protein affinity reagents. This unit describes the synthesis and purification of POC molecules using the heterobifunctional crosslinking reagent succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC), which enables amine-modified oligonucleotides to become covalently linked to cysteine-modified polypeptides. This solution-based protocol consists of a two-step synthesis followed by a single purification step.

Source: Current Protocols in Nucleic Acid Chemistry

DOI: 10.1002/0471142700.nc0441s42

Authors: Berea A.R. Williams, John C. Chaput

Institution: Arizona State University

Language: English

Topic: Chemical Synthesis and Analysis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Cancer Institute

Awards: G1433160883, A High-throughput Route to Synthetic Antibodies for Array-based Cancer Detection