Work

On the Mechanism of the Pentose Phosphate Epimerases

Year: 1972

Type: article

Abstract: The mechanism of the l-ribulose 5-phosphate 4-epimerase has been examined. In agreement with previous observations (Mcdonough, M. W., and Wood, M. A. (1961) J. Biol. Chem. 236, 1220) no exchange of hydrogen with H2O is observed. Suitable double label experiments indicate that hydrogen transfer in the reaction is intramolecular. The data for kinetics of the exchange of the hydrogen at C-3 of substrate catalyzed by a d-ribulose 5-phosphate 3-epimerase have been examined. The data indicate that the enzyme must contain two hydrogen donors (acceptors) which interact with the hydrogen at carbon 3 of d-ribulose 5-phosphate and d-xylulose 5-phosphate, respectively.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(19)44837-0

Authors: Linda Davis, Nancy Lee, Luis Glaser

Institutions: University of California, Santa Barbara, Washington University in St. Louis

Language: English

Topic: Chemical Reaction Mechanisms

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1562383182

FWCI: 0.664

Cites: 21

Cited by: 28

Related to: 10