Work

DNA-directed in vitro synthesis of Escherichia coli beta-isopropylmalate dehydrogenase.

Year: 1978

Type: article

Abstract: The in vitro synthesis of beta-isopropylmalate dehydrogenase (EC 1.1.1.85), an enzyme involved in leucine biosynthesis, has been obtained using as template DNA from the hybrid plasmid (pLC1) which contains the Escherichia coli leucine operon. Enzyme synthesis in vitro is stimulated about 2-fold by guanosine-5'-diphosphate-3'-diphosphate and inhibited about 60% by 2 X 10(-4) M L-leucine.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(17)38041-9

Authors: H. Kung, H. Weissbach

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1563390956

FWCI: 0.188

Cites: 17

Cited by: 4

Related to: 10