Work

Isolation and Characterization of the nikR Gene Encoding a Nickel-Responsive Regulator in Escherichia coli

Year: 1999

Type: article

Abstract: Expression of the nickel-specific transport system encoded by the Escherichia coli nikABCDE operon is repressed by a high concentration of nickel. By using random transposon Tn10 insertion, we isolated mutants in which expression of the nik operon became constitutive with respect to nickel. We have identified the corresponding nikR gene which encodes a nickel-responsive regulator. Expression of nikR was partially controlled by Fnr through transcription from the nikA promoter region. In addition, a specific transcription start site for the constitutive expression of nikR was found 51 bp upstream of the nikR gene.

Source: Journal of Bacteriology

DOI: 10.1128/jb.181.2.670-674.1999

Authors: Karinne De Pina, Valerie Desjardin, Marie-Andree Mandrand-Berthelot, Gerard Giordano, Long-Fei Wu

Institutions: Centre National de la Recherche Scientifique, Laboratoire de Chimie Bactérienne

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W1563527550

FWCI: 2.572

Cites: 29

Cited by: 184

Related to: 10