Work

Biosynthesis of the Peptidoglycan of Bacterial Cell Walls

Year: 1967

Type: article

Abstract: The particulate enzyme preparation from cells of Micrococcus lysodeikticus which catalyzes peptidoglycan synthesis contains both enzyme proteins and an essential phospholipid which acts as a carrier of sugar fragments during peptidoglycan synthesis. This enzyme preparation has been separated into its protein and lipid components by extraction with chloroform-methanol (1:1) at -17°. The enzyme protein and phospholipid have been reconstituted in the presence of deoxycholate at low concentrations. The endogenous phospholipid acceptors have been labeled with 32P and then separated from other 32P-phospholipids by thin layer chromatography in several solvents. Two 32-P-phospholipid fractions were separated, each of which could be converted to the 32P-phospholipid intermediate, disaccharide(-pentapeptide)-P-P-phospholipid, in the presence of uridine diphosphate acetylmuramyl-pentapeptide and UDP-acetylglucosamine.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(18)95952-1

Authors: Carl P. Dietrich, Anthony V. Colucci, Jack L. Strominger

Institution: University of Wisconsin–Madison

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1565077215

FWCI: 2.123

Cites: 8

Cited by: 22

Related to: 10