Work

Cutting Edge: Senescent BALB/c Mice Exhibit Decreased Expression of λ5 Surrogate Light Chains and Reduced Development Within the Pre-B Cell Compartment

Year: 1998

Type: article

Abstract: Although senescent BALB/c mice (approximately 2 years old) have reduced numbers of small pre-B cells, early pre-B cells (CD43+CD25+B220+) are present in comparable numbers within the bone marrow of both young (3-6-month-old) and senescent BALB/c mice. The transition of CD43+ pre-B cells to the CD43- pre-B cell compartments is dependent on proliferation and clonal maturation dictated by the pre-B cell receptor (mu/lambda5/VpreB). In vivo, senescent CD43+B220+ pro-B/early pre-B cells demonstrated reduction of lambda5 mRNA, by RT-PCR analysis, and of both surface and cytoplasmic lambda5 protein. Decreased lambda5 protein expression was also seen among pro-B/pre-B cells derived from senescent bone marrow after stimulation in vitro with IL-7. We propose that diminished expression of the lambda5 surrogate light chain results in decreased pre-B cell receptor formation and contributes to reduced recruitment of nascent CD43+ pre-B cells into the CD43- large and small pre-B cell compartments.

Source: The Journal of Immunology

DOI: 10.4049/jimmunol.161.9.4472

Authors: Erin M Sherwood, Bonnie B Blomberg, Wei Xu, Cynthia A Warner, Richard L Riley

Institution: University of Miami

Language: English

Topic: T-cell and B-cell Immunology

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: Sylvester Comprehensive Cancer Center, University of Miami Health Systems, National Institute on Aging