Work

Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.

Year: 1981

Type: article

Abstract: A method is described for the affinity purification of antibodies using protein samples that have been electrophoretically transferred to diazotized paper. Using differentiated neuroblastoma cells as the protein sample and a heterogeneous anti-microtubule protein serum, antibodies were isolated that specifically bound only to tubulin on blots and that stained microtubule networks in cells. The general utility of this method for various types of applications is discussed.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(18)43211-5

Author: J.B. Olmsted

Language: English

Topic: Monoclonal and Polyclonal Antibodies Research

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1566845757

FWCI: 6.508

Cites: 10

Cited by: 792

Related to: 10