In Vivo Optical Imaging of Fluorescent Markers for Detection and Guided Resection of Cancer
Year: 2006
Type: dissertation
Abstract: Fluorescent molecules, hereafter referred to as fluorophores, can be excited by \nillumination with light and subsequently can de-excite into the ground state through, \namongst others possibilities, emission of fluorescence. In case the fluorophore is selectively \ntaken up by the cancer and not by surrounding normal tissues the fluorescence may thus be \nused for the detection and localization of cancers. \n\n \n\nThe research described in this thesis focuses on the development of methods and \ntechnology for real-time in vivo fluorescence imaging for cancer detection and image-guided \nsurgical tumor resection. Subsequently, the introduced methods and technology are \nevaluated through modelling studies and validated with pre-clinical experiments and clinical \ntrials in patients. \n\n \n\nAfter a general introduction in Chapter 1, Chapter 2 describes the development of a \nfluorescence imaging device for the detection of superficial cancer based on the Double \nRatio technique. In practical use this device resembles an operation microscope and can be \nused in a clinical environment. This device acquires 4 different fluorescence images excited \nat two wavelengths each detected at two wavelengths. From these images it calculates, \ndisplays and stores Double Ratio images at a maximum speed of 1Hz. The Double Ratio \nimage gives the distribution of the fluorophore amount present in tissue and is not affected \nby local variations in tissue optics, i.e. tissue absorption and tissue scattering. The validity of \nthe technique was confirmed here by ex vivo tissue equivalent phantom experiments using \nhematoporphyrin and in vivo experiments on normal pigmented moles on Caucasian human \nskin using aminolevulinic acid \n\n \n\nIn Chapter 3 we investigated if the device described in Chapter 2 could be used as an \nimaging technique for the diagnoses of cervical intraepithelial neoplasia (CIN) in patients.
Author: Arjen Bogaards
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W1567096412