A Comparison of Estimates of Michaelis-Menten Kinetic Constants from Various Linear Transformations
Year: 1965
Type: article
Abstract: The parameters which characterize this equation, and which must ordinarily be estimated from the observed data, are ‘v,,,, the maximum initial velocity which is theoretically attained when the enzyme has been “saturated” by an infinite concentration of substrate, and K,, the Michaelis constant which is numerically equal to the concentration of substrate for half-maximal initial velocity. Since the relationship between the independent variable, Cs, and the dependent variable, v, is curvilinear, it has long been customary to facilitate estimation of the two parameters by plotting the experimental data according to one of the following three linear transformations of Equation 1. v = Vmax - K&/C’s) (la) (Cs/v) = (Km/Vm,x) + (l/Vm,x)Cs (lb)
Source: Journal of Biological Chemistry
Authors: John E. Dowd, Douglas S. Riggs
Institution: University at Buffalo, State University of New York
Language: English
Subfield: Physical and Theoretical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W1567610244