Polynucleotide-dependent Incorporation of Amino Acids in a Cell-free System from Thermophilic Bacteria
Year: 1966
Type: article
Abstract: The preparation of a cell-free amino acid incorporation system from Bacillus stearothermophilus active at 65° and the conditions for optimal activity with synthetic polynucleotide messengers are described. The activity of this system is comparable to that of the conventional Escherichia coli system. Its thermal stability, high activity, and low nuclease content are convenient features for studies of protein synthesis and coding. Thus, a 1.4:1 copolymer of adenylic and uridylic acids, which, because of a high degree of ordered structure, is almost inactive in the E. coli system at 37°, promoted significant incorporation of isoleucine, leucine, and lysine in the B. stearothermophilus system at 65°.
Source: Journal of Biological Chemistry
Authors: Israel D. Algranati, Peter Lengyel
Institution: New York University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W1568998944