Determination of the distance between the oligosaccharyltransferase active site and the endoplasmic reticulum membrane.
Year: 1993
Type: article
Abstract: By in vitro transcription/translation of model proteins in the presence of dog pancreas microsomes, we have measured the minimum distance of an acceptor site from the lumenal end of a transmembrane segment required for N-linked glycosylation, both when the acceptor site is placed N- and C-terminally to the membrane anchor. We observe a sharp threshold at a distance of 12-14 residues, suggesting that the oligosaccharyltransferase active site is 30-40 A above the membrane and is oriented roughly parallel to the membrane surface.
Source: Journal of Biological Chemistry
Authors: I.M. Nilsson, G. von Heijne
Institution: Karolinska Institutet
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W1571379445