Work

DNA Probes Using Fluorescence Resonance Energy Transfer (FRET): Designs and Applications

Year: 2001

Type: review

Abstract: Fluorescence resonance energy transfer (FRET) is widely used in biomedical research as a reporter method. Oligonucleotides with a DNA backbone and one or several chromophore tags have found multiple applications as FRET probes. They are especially advantageous for the real-time monitoring of biochemical reactions and in vivo studies. This paper reviews the design and applications of various DNA-based probes that use FRET The approaches used in the design of new DNA FRET probes are discussed.

Source: BioTechniques

DOI: 10.2144/01315rv02

Author: Vladimir V. Didenko

Institution: Baylor College of Medicine

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,415

Funders: Texas Higher Education Coordinating Board, Karolinska Institutet, National Institute on Aging