Modification of the IFCC reference measurement procedure for determination of HbA 1c by HPLC-ESI-MS.
Year: 2006
Type: article
Abstract: The reference measurement procedure for determination of HbA(1c) (glycated haemoglobin) using HPLC(high performance liquid chromatography)-ESI(electrospray ionisation)-MS(mass spectrometry) has been modified. Main modifications were a change in the buffer composition of the HPLC, a change in the gradient elution profile and the introduction of a post-column splitting system. The long-term stability of the HPLC-ESI-MS system proved to be of high importance to get reproducible results. Using the modified HPLC conditions stable retention times, high peak symmetry and a good column batch-to-batch reproducibility of the analytical results were obtained. The mean deviation from the target values was less than 0.7% with an imprecision of less than 2%.
Source: Europe PMC (PubMed Central)
Authors: Patricia Kaiser, Theodorus Akerboom, László Dux, Hans Reinauer
Institution: Instand
Language: English
Subfield: Medical Laboratory Technology
Field: Health Professions
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W1577250543