Nonradioactive PCR-enzyme-linked immunosorbent assay method for detection of human cytomegalovirus DNA
Year: 1995
Type: article
Abstract: We developed a rapid, sensitive, and specific PCR-based assay for human cytomegalovirus (HCMV). The assay includes primer and probe sequences derived from conserved HCMV nucleotide sequences and nonradioactive hybridization-confirmation. The assay detected between 10 and 100 viral genomes. All HCMV clinical isolates tested (39 of 39) gave positive reactions.
Source: Journal of Clinical Microbiology
Authors: R D Allen, P E Pellett, J A Stewart, M Koopmans
Institution: Centers for Disease Control and Prevention
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W1578065547