Work

Spectrophotometric Determination of the Dissociation Constant of Ribonuclease S'

Year: 1968

Type: article

Abstract: Spectrophotometric determination of the dissociation constant of ribonuclease S' at low ionic strength and in the absence of substrate yields values for Kd three to four orders of magnitude larger than those determined by using recovery of activity as the criterion for association. These values correspond with calculations from earlier data from another laboratory, and seem reasonable for a peptide-protein interaction involving noncovalent bonds.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(18)93599-4

Authors: B M Woodfin, V Massey

Institution: University of Michigan

Language: English

Topic: Protein Structure and Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1578212106

FWCI: 0.651

Cites: 16

Cited by: 26

Related to: 10