Biological control of apple scab and fire blight by the application of the non-pathogenic bacterium Pseudomonas fluorescens Bk3 to the leaf surface.
Year: 2008
Type: article
Abstract: The biological control of plant diseases by application of antagonistic microorganisms to \nthe plant phyllosphere is an alternative strategy to prevent the frequent treatment of plants \nby pesticides. Microbiological antagonists can firstly interact directly against the pathogen \nby releasing antimicrobial compounds and/or secondly induce the plant resistance of the \nhost plant by expression of pathogenesis-related proteins (PR proteins). The focus of our \nstudy is on the interaction of the non-pathogenic bacterium Pseudomonas fluorescens Bk3 \nto the plant phyllosphere of Malus domestica cv. Holsteiner Cox. After application of P. \nfluorescens Bk3 to the phyllosphere of M. domestica cv. Holsteiner Cox we observed \ndramatic changes in the protein composition of the apoplast of the host plant. Sequencing \nof the induced proteins by ESI-Q-ToF mass spectrometry and homology search identified \nthese additional proteins as pathogenesis related proteins (PR) like ß-1,3- glucanase, \nthaumatin-like protein, chitinase and hevein-like protein. To confirm these findings, a \nsuppressive subtractive hybridization with total RNA from leaves before and after \ninoculation of P. fluorescens Bk3 to the leaves of the host plant was performed. It revealed \nan increased expression level of many PR and stress related genes. \nThe induction of PR proteins and plant defence genes in host plants after application of \nnon-pathogenic bacterial antagonists to the plant phylloshere can presumably prevent or \nreduce successful infections by plant pathogens.
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
Funder: Deutsche Bundesstiftung Umwelt
OpenAlex ID: W1578216602