Work

Transforming growth factor beta regulates the metabolism of proteoglycans in bovine cartilage organ cultures.

Year: 1988

Type: article

Abstract: The effect of transforming growth factor beta (TGF-beta) has been studied in a bovine articular cartilage organ culture. The peptide stimulates synthesis of proteoglycans in a dose-dependent manner, reaching saturation at 10 ng/ml. This dose gave an approximate 7-fold increase in synthesis over basal controls. In addition, the peptide decreased the rates of catabolism of proteoglycans with an approximately 2-fold maximal effect seen at 5 ng/ml. At the latter concentration, TGF-beta prevented the 4-fold loss of proteoglycans which occurred in cultures maintained under basal conditions over the course of 3 weeks. There was no increase in cell (DNA) content of the cartilage explants under these conditions of TGF-beta treatment, and the net collagen content of the explants remained constant.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(18)37634-8

Authors: T I Morales, A B Roberts

Institutions: National Institutes of Health, National Institute of Dental and Craniofacial Research

Language: English

Topic: Osteoarthritis Treatment and Mechanisms

Subfield: Rheumatology

Field: Medicine

Domain: Health Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1582355914

FWCI: 13.36

Cites: 18

Cited by: 272

Related to: 10