Work

Binding of single-chain prourokinase to the urokinase receptor of human U937 cells.

Year: 1986

Type: article

Abstract: The single-chain form of human urokinase plasminogen activator (uPA) is the major form of the enzyme found in cells, tissues, and extracellular fluids. The protein, called pro-uPA, has high (Kd = 0.5 nM) affinity for the specific uPA receptor of U937 human monocyte-like cells. Its conversion to two-chain uPA by plasmin does not appreciably change the binding parameters. In addition, conversion of pro-uPA to uPA occurs with receptor-bound pro-uPA and does not lead to dissociation from the membrane. These data show that secreted pro-uPA can find its way to the specific surface receptor without previous conversion to the two-chain form and that, once bound, can be activated by plasmin.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(18)66635-9

Authors: M V Cubellis, M L Nolli, G Cassani, F Blasi

Institutions: Institute of Genetics and Biophysics, National Research Council

Language: English

Topic: Protease and Inhibitor Mechanisms

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1584486859

FWCI: 5.837

Cites: 33

Cited by: 188

Related to: 10