Year: 2011
Type: dissertation
Abstract: In this research, comparative studies of planktonic and biofilm modes of growth in Salmonella enterica serovar Typhimurium were undertaken.Chemical disinfectant studies were performed for both planktonic and biofilm Salmonella and biofilms at different ages.A gas chromatography coupled to mass spectrometry (GC-MS) metabolite profiling approach was used to evaluate metabolic variations between planktonic and biofilm Salmonella and biofilms at different ages.Biofilm growth curves were generated to assess their ability to grow as a biofilm in the minimum biofilm eradication concentration (MBEC) assay system, and to achieve the most stable biofilm to carry out disinfectant susceptibility testing.Growth curves demonstrated that biofilms grown in nutrient poor conditions produced a stable biofilm over a seven-day growth period and as such, were used for susceptibility testing.Results showed that Salmonella biofilms were more resistant to disinfectants compared to planktonic Salmonella, but increasing the age of a biofilm did not increase resistance to disinfectants.By using a GC-MS metabolomics approach, growth media were compared with supernatants from planktonic and biofilm cultures (extracellular metabolites); and biofilm cell extracts were compared with those from planktonic cells (intracellular metabolites).Principal component analysis (PCA) and spectral comparisons revealed differences between the overall metabolic profiles of planktonic and biofilm S.Typhimurium.The metabolic profiles of biofilms at different ages were subsequently obtained and evaluated.PCA and spectral comparisons revealed no significant iv differences in intracellular metabolite profiles between biofilms of different ages.On the other hand, PCA analysis showed that the extracellular metabolite profiles of biofilms were different between the different ages of biofilm supernatant.In conclusion, biofilms were more resistant to disinfectants compared to planktonic Salmonella, but this resistance was not increased in older biofilms.Metabolic analysis shows that there exist chemical differences between planktonic and biofilm modes of growth in Salmonella.Finally, there were differences in the extracellular metabolite profiles of biofilms at different ages, however such differences were not observed in intracellular metabolite profiles of Salmonella Typhimurium.vACKNOWLEDGEMENTS First and foremost I would like to thank my supervisors, Ryan O'Handley, Rob Trengove, Garth Maker and Stan Fenwick.Ryan, here is to the chilli, dim sum, and Moets.Three wordsbest supervisor ever!Rob, to the coffees and desserts; Garth, to the pharmacy labs and super brainpower and Stan, for your words of wisdom, and creative ideas.I would also like to thank Kirsty Townsend
Author: Hui San Wong
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funder: Murdoch University
OpenAlex ID: W1584519865