Work

Repair of O6-Methylguanine by ABC Excinuclease of Escherichia coli in vitro

Year: 1989

Type: article

Abstract: O6-Methylguanine, the major mutagenic product of methylnitroso compounds, was previously thought to be repaired exclusively by alkyltransferases I and II. Using synthetic substrates that contain O6-methyl-guanine at defined positions, we demonstrate that the nucleotide excision repair enzyme of Escherichia coli, ABC excinuclease, also repairs DNA containing this adduct. We show that the ABC excinuclease binds specifically to the modified DNA and produces incisions at the eight phosphodiester bond 5' and at the fifth or sixth phosphodiester bond 3' to the modified guanine.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(18)83715-2

Authors: J M Voigt, B Van Houten, A Sancar, M D Topal

Institution: University of North Carolina at Chapel Hill

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

Funders: National Cancer Institute, National Institute of General Medical Sciences