Work

Production of Shotgun Libraries Using Random Amplification

Year: 2001

Type: article

Abstract: In the following report, thermal cycling coupled with random 10-mers as primers was used to construct randomly amplified shotgun libraries (RASLs). This approach allowed shotgun libraries to be constructed from nanogram quantities of input DNA. RASLs contained inserts from throughout a target genome in an unbiased fashion and did not appear to contain chimeric sequences. This protocol should be useful for shotgun sequencing the genomes of unculturable organisms and rapidly producing shotgun libraries from cosmids, fosmids, yeast artificial chromosomes (YACs), and bacterial artificial chromosomes (BACs).

Source: BioTechniques

DOI: 10.2144/01311rr02

Authors: F. Rohwer, V. Seguritan, D.H. Choi, A.M. Segall, F. Azam

Institutions: Scripps Institution of Oceanography, San Diego State University, Seoul National University

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,415

Funders: National Science Foundation, San Diego State University, National Institutes of Health, National Institute of General Medical Sciences