Work

Multiphoton Redox Ratio Imaging for Metabolic Monitoring In Vivo

Year: 2009

Type: book-chapter

Abstract: Metabolic monitoring at the cellular level in live tissues is important for understanding cell function, disease processes, and potential therapies. Multiphoton imaging of the relative amounts of NADH and FAD (the primary electron donor and acceptor, respectively, in the electron transport chain) provides a noninvasive method for monitoring cellular metabolic activity with high resolution in three dimensions in vivo. NADH and FAD are endogenous tissue fluorophores, and thus this method does not require exogenous stains or tissue excision. We describe the principles and protocols of multiphoton redox ratio imaging in vivo.

Source: Methods in molecular biology

DOI: 10.1007/978-1-60761-411-1_11

Authors: Melissa Skala, Nirmala Ramanujam

Institution: Duke University

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Cancer Institute, National Institute of Biomedical Imaging and Bioengineering